Advances in Mass Data Analysis of Signals and Images in by Petra Perner, Ovidio Salvetti

By Petra Perner, Ovidio Salvetti

This ebook constitutes the refereed court cases of the foreign convention on Mass information research of signs and pictures in drugs, Biotechnology and Chemistry, MDA 2006 and 2007, held in Leipzig, Germany..

The issues comprise options and advancements of sign and picture generating tactics, item matching and item monitoring in microscopic and video microscopic pictures, 1D, 2nd and 3D form research, description and have extraction of texture, constitution and placement, picture segmentation algorithms, parallelization of photograph research and semantic tagging of pictures from existence technological know-how applications.

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Thus, pixels characterized by gray-level g, g<θi+k×δ, possibly present within a darker component are not assigned to the background, so that spurious background components are not created. Another advantage of removing pixels by our method is that we can record the list L of removed pixels in the order they have been removed. This will play a useful role during topological expansion. In Fig. 2, the images resulting after the 5-th iteration and the 6-th iteration of nontopological erosion are shown, where black and gray respectively denote the seeds and the pixels (temporarily) assigned to the background.

The main advantages of the proposed method are its abilities to segment images where the sizes of the object and background pixels are heavily unequal, and to assign pixels which are scatteredly distributed over the whole image as the background ones. This type of images are very common in cellular and molecular imaging where the regions of interest occupy only a very small part of the image, and the staining effect is usually scattered over the whole image space. Regarding the computational time, for an image size of 1040 × 1392, runnning Matlab codes on an average PC, it took about one second for Otsu thresholding; a few seconds for the watershed method, twelve minutes for the FCM-based segmentation; and about seven and two minutes for the variogram-based thresholding taking five (h=1, .

1 Non-topological Erosion The range of gray-levels between θi and θf is sampled into n groups. Each group gathers δ successive gray-levels. In our case, it is δ=5. This choice is motivated by a compromise in computational efficiency (the larger is δ, the smaller is n and, hence, the number of iterations of the process) and quality of the result (with a small δ, also perceived foreground components which are remarkably lighter with respect to other foreground components can be singled out. In turn, if δ is large, lighter foreground components risk to be lost, since no seeds would be detected in correspondence with them at any iteration to allow their recovery after non-topological erosion).

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